There is Three ways to Enable/disable USB port in windows

First Method

• Right click on MY COMPUTER,
• then select MANAGE option. a new window will appear, in that
• select SYSTEM TOOLS, in that
• click on DEVICE MANAGER, on the right side of the window pane.
• select the USB port,  right click and diable/enable  the port

Second Method

• disable through Bios

Third Method

1. Click Start, and then click Run.
2. type regedit, and then click OK.
3. Locate, and then click the following registry key: HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Services\UsbStor
4. In the right pane, double-click Start.
5. In the Value data box, type 4 (4 to disable, 3 to Enable) , and then click OK.
6. Quit Registry Editor.